Figure 1.

Study design. Cartilage constructs with central defects were generated through dissection of articular cartilage to model a confined cartilage defect. The central defects were grafted with a BioCartilage/BMC mixture which was sealed with 1 of 5 types of fibrin glue to model cartilage repair. Repair constructs were cultured for 24 or 48 hours then imaged using live/dead confocal microscopy and SEM. For SEM, cartilage defects were also grafted with isolated bone marrow-derived MSC or articular chondrocytes. IL-1RA was measured in medium and biologics (PRP, PPP, BMC, commercial fibrin glue). Proteomics was performed on BioCartilage. BMC, bone marrow concentrate; SEM, scanning electron microscopy; PRP, platelet-rich plasma; PPP, platelet-poor plasma; IL-1RA, interleukin-1 receptor antagonist. Letters in parentheses denote source: H, human; E, equine.