TABLE 1.

Sequences of primers and probes used in this study^a

Gene and primer or probe	Sequence
Universal 18S rRNA
Sense primer	5′CGGCTACCACATCTAAGG 3′
Antisense primer	5′TATACGCTATTGGAGCTGG 3′
Toxo ITS1
Sense primer	5′GATTTGCATTCAAGAAGCGTGATAGTA-t 3′
Antisense primer	5′AGTTTAGGAAGCAATCTGAAAGCACATC 3′
Probe	5′(6-FAM)-CTGCGCTGCTTCCAATATTGG-(TAMRA) 3′
Mouse (Hu) beta-actin
Sense primer	5′TCACCCACACTGTGCCCATCTACGA-3′
Antisense primer	5′CAGCGGAACCGCTCATTGCCAATGG 3′
Probe	5′(6-FAM)-ATGCCC-x (TAMRA)-CCCCATGCCATCCTGCGT 3′

^ax, TAMRA dye coupling site.