TABLE 2.

Toxo TaqMan results for samples from T. gondii-infected pigs and mice^a

Animal no.	T. gondii strain	Inoculumb	Antibodies to T. gondii (titer)	CT
				T. gondii	Housekeeping gene	NormalizedcT. gondii
M 7076	VEG	200 B	≥1:50	24.69	25.86	21.96
M 7077	VEG	200 B	≥1:50	24.51	27.84	23.46
M 7078	VEG	200 B	≥1:50	25.49	28.16	24.68
M 7079	VEG	200 B	≥1:50	27.2	27.64	25.85
M 7080	VEG	200 B	≥1:50	25.05	27.82	23.96
M 8348	Me49	1 T	≥1:200	28.84	28.84	28.44
M 8349	Me49	1 T	≥1:200	29.41	28.23	29.19
M 8350	Me49	1 T	≥1:200	29.39	27.25	28.16
M 8351	Me49	1 T	≥1:200	29.19	27.31	28.03
MP 521	VEG	500 O	1:800	32.26	N/D	N/D
MP 522	VEG	500 O	1:1,600	30.33	N/D	N/D
MP 523	VEG	500 O	1:400	32.24	N/D	N/D
MP 524	VEG	500 O	1:200	33.52	N/D	N/D
MP 526	VEG	500 O	1:800	32.09	N/D	N/D
MP 490	None	None	<1:25	≥50	N/D	N/D
MP 532	None	None	<1:25	≥50	N/D	N/D

^aDNA samples from Swiss-Webster mice (M) (500 ng, total input) and NIH minipigs (MP) (1 μg) infected with different T. gondii strains were analyzed by the Toxo TaqMan assay. DNA was obtained from brain tissues as described in Materials and Methods. Tachyzoites and bradyzoites were injected subcutaneously, and oocysts were orally inoculated. Specific antibody detection was performed by MAT as described in Materials and Methods. 

^bB, counted bradyzoites; T, tachyzoites, determined by bioassay in mice (the 10⁻⁵ dilution was infective to all four mice, the 10⁻⁶ dilution was not infective to any of the four mice); O, live oocysts, determined by dilution bioassay in mice. 

^cNormalization factor: T. gondii C_T value × (β-actin C_T sample/highest β-actin C_T value). N/D, not determined.