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. 2022 Jan 20;25(3):96. doi: 10.3892/mmr.2022.12612

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — 3-MA reverses the anti-inflammatory and anti-apoptotic effects of hydrogen sulfide on HK-2 cells by inhibiting autophagy. (A) The expression of LC3B and p62 proteins in HK-2 cells after 3-MA treatment. (B) The ratio of p62/β-actin. (C) The ratio of LC3B-II/LC3B-I. (D) Immunofluorescence analysis of LC3B punctate aggregation in cells. Scale bar, 20 µm. (E) Statistics of the average number of autophagosomes in each group. (F) Level of IL-1β in cell supernatant after 3-MA treatment. (G) Level of IL-18 in cell supernatant after 3-MA treatment. (H) The apoptosis rate detected by flow cytometry after 3-MA treatment. (I) Statistics of apoptosis index. Data are presented as the mean ± SD and represent three independent experiments. 3-MA, 3-methyladenine; LC3B, microtubule associated protein 1 light chain 3; IL, interleukin; NaHS, sodium hydrosulfide hydrate; LPS, lipopolysaccharide.