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. 2021 Nov 8;77(2):320–330. doi: 10.1093/jac/dkab393

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© The Author(s) 2021. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Schematic diagram showing the structural organization of vanA transposon regions closed by hybrid assembly from VREfm isolates from Irish hospitals. (a) SJ10 (SJ10vanA, 11 022 bp); (b) SJ11 (SJ11vanA, 13 252 bp); (c) BSI_SJ40 (BSI_SJ40vanA, 13 269 bp); (d) SJ82 (10 823 bp vanA region from plasmid pSJ82vanA); (e) SJ245 (14 168 bp vanA region from plasmid pSJ245vanA); (f) RC_19_039 (13 601 bp vanA region from plasmid pRC_19_039); (g) RC_19_023 (RC_19_023vanA, 12 883 bp); and (h) the structural organization of the prototype vanA transposon Tn1546 from VREfm BM4147 (GenBank accession number: M97297). VREfm isolates were recovered from H1 (a–e), H3 (f) and H4 (g). Genes and their orientation are denoted by directional arrows and labelled with corresponding gene names. A reference size scale bar is shown at the bottom of the diagram. The extent of the transposase tnpA gene sequences deleted (Δ) in the various assemblies is shown in Figure 4.