Figure 5.

Schematic diagrams of the structural organization of (a) plasmids pSJ82_B and pSJ82vanA from VREfm donor strain SJ82, and (b) the vancomycin resistance plasmid pSJ82_TC from the transconjugant SJ82:Efm 64/3 TC1. Diagrams are partially annotated for clarity. Genes of interest and their orientation are represented by arrows: red indicates antibiotic resistance genes, orange indicates ISs/transposases and blue indicates genes encoding known proteins. The regions from plasmids resolved by hybrid assembly of paired-end Illumina MiSeq short reads with Oxford Nanopore Technologies long reads present in the donor isolate SJ82 (i.e. pSJ82_B and pSJ82vanA) that are present in the hybrid transconjugant plasmid pSJ82_TC are marked as follows: pSJ82_B region highlighted in blue and pSJ82vanA region highlighted in red. Plasmid regions in pSJ82vanA and pSJ82_B shaded in black were not present in pSJ82_TC.