FIG. 1.

Map of M. leprae folP1 SRDR. Wild-type is the nucleic acid sequence of folP1 SRDR of DDS-susceptible M. leprae Thai-53. The underlined bases indicate the PCR primers for amplification of a 231-bp SRDR fragment (SRDR-F and -R) and a 144-bp UHG-DDS-144 generator (SRDR-F and UHG-144-R). Mutant alleles are those mutations found in DDS-resistant strains; UHG-DDS-144 is the 144-bp UHG containing two 3-bp deletions (∗∗∗) and used in the HD-DDS-ML assay to enhance the detection of mutant alleles associated with DDS resistance in M. leprae.