TABLE 1.
M. leprae strains used in this study
| M. leprae strain | Sample typea | Origin | DDS susceptibilityb | PCR resultsc | Mutant alleled | HDA-DDS-ML profilee |
|---|---|---|---|---|---|---|
| 2262 | HSB | United States | >0.01 | + | Thr53Ala (ACC→GCC) | R |
| CDC-DDS-1 | MFP | United States | >0.01 | + | Thr53Ala (ACC→GCC) | R |
| 61-00 | MFP | India | >0.01 | + | Thr53Ala (ACC→GCC) | R |
| India-2, 63-00 | MFP | India | >0.01 | + | Thr53Arg (ACC→AGG) | R |
| 2898 | HSB | United States | >0.01 | + | Thr53Ile (ACC→ATC) | R |
| Airaku-3, Zensho-4 | MFP | Japan | >0.01 | + | Thr53Ile (ACC→ATC) | R |
| 591 | MFP | Nepal | >0.01 | + | Pro55Arg (CCC→CGC) | R |
| India-4, 62-00 | MFP | India | >0.01 | + | Pro55Arg (CCC→CGC) | R |
| 64-00 | MFP | India | >0.01 | + | Pro55Leu (CCC→CTC) | R |
| Zensho-2 | MFP | Japan | >0.01 | + | Pro55Leu (CCC→CTC) | R |
| SA26 | HSB | United States | >0.01 | + | None | S |
| 569 | MFP | Nepal | >0.001 and <0.01 | + | None | S |
| H101, H103, H107, NIH-1 | HSB | United States | >0.001 and <0.01 | + | None | S |
| Thai-53 | MFP | Thailand | <0.0001 | + | None | S |
| 19-F-1 | ALN | Philippines | <0.0001 | + | None | S |
| H105 | HSB | United States | <0.0001 | + | None | S |
a
HSB, human skin biopsy specimen; ALN, armadillo lymph node.
b
DDS susceptibility in MFP assay (<0.0001% DDS as a percentage of food is considered susceptible).
c
+, DNA template generates a 231-bp folP1 fragment and a 360-bp 18-kDA gene fragment (8).
d
Mutant alleles in the SRDR of folP1 obtained by PCR and automated DNA sequencing.
e
Heteroduplex analysis results: S, DDS-susceptible profile; R, DDS-resistant profile.