TABLE 2.
Plasmids used in this study
| Plasmid | Feature(s) | Source or reference |
|---|---|---|
| pUC19 | pUC, bla | 33 |
| pUCsRNAP | pUC, bla; small RNA promoter and 23-bp spacer sequence flanked by two 19-bp repeats | This study |
| pRPF185 | Tetracycline-inducible expression plasmid | 34 |
| pDL-1 | pMTL82151 backbone with Cas12a under control of Ptet promoter from pRPF185 | Gift from Duolong Zhu, Baylor College of Medicine |
| pVPL3004 | Emr derivative of pNZ9530 | 24 |
| pJC005.em | Emr CRISPR-Cas12a genome editing plasmid | This study |
| pJC005.gent | Gmr CRISPR-Cas12a genome editing plasmid | This study |
| pJC005.em.XacpH | Emr plasmid targeting clean deletion of acpH | This study |
| pJC005.em.XlacL | Emr plasmid targeting clean deletion of lacL | This study |
| pJC005.em.XtreA | Emr plasmid targeting clean deletion of treA | This study |
| pJC005.em.XtreA::unaG | Emr plasmid targeting clean knock-in of unaG in place of treA | This study |