TABLE 2.
Host range and efficiency of plating (EOP) of phage Sa45lw against various Shiga toxin-producing Escherichia coli (STEC) and Salmonella enterica strains
| Strains | Strain ref. no. | EOPa |
|---|---|---|
| STEC O26 | E. coli O26:H− (RM18132), E. coli O26:H− (RM17133) | R* |
| STEC O45 | E. coli O45:H− (RM10729) | 0.39 |
| E. coli O45:H16 (RM13752) | 0.56 | |
| Clinical E. coli O45:H2 (SJ7), E. coli O45:H2 (96-3285) | R | |
| STEC O103 | E. coli O103:H− (RM10744), E. coli O103:H2 (RM13322) | R |
| STEC O111 | E. coli O111:H− (RM11765), E. coli O111:H− (RM14488) | R |
| STEC O121 | E. coli O121:H19 (96-1585), E. coli O121:H− (RM8082) | R |
| STEC O145 | E. coli O145:H+ (RM9872), E. coli O145:H- (RM10808) | R |
| STEC O157 | E. coli O157:H7 (RM18959) | 0.17 |
| E. coli O157:H7 (ATCC 35150) | 0.19 | |
| E. coli O157:H7 (ATCC 43888) | 0.76 | |
| Generic E. coli | ATCC 13706 | H^ |
| DH5α | R | |
| Salmonella | Salmonella Montevideo | 0.11 |
| Salmonella Newport | R | |
| Salmonella Heidelberg | R | |
| Salmonella Enteritidis | R | |
| Salmonella Typhimurium | R | |
| Salmonella Thompson | 1.05 | |
| Salmonella Anatum | <0.001 |
a
EOP was conducted on the spot test-positive strains and is presented with a value that was calculated by the ratio of phage titer on test bacterium relative to the phage titer on the primary bacterium used for isolation. High production efficiency is EOP ≥ 0.5, medium production efficiency is 0.5 > EOP ≥ 0.1, low production efficiency is 0.1 > EOP > 0.001, and the inefficiency of phage production is EOP ≤ 0.001. * R denotes no lysis from the spot test assay. ^H was the primary bacterial strain used for isolation.