Fig. 5. RAPA@Lipo@HMs maintain cellular homeostasis.
(A) Intracellular ROS generation measured by DCF. (B) Cell apoptosis measured by TUNEL staining. (C) Cell death measured by Live/Dead assay. (D) Quantitative analysis of ROS expression based on DCF fluorescence intensity. (E) Quantitative analysis of apoptotic cell rate based on TUNEL fluorescence intensity. (F) Dead cell percentage obtained from the Live/Dead staining assay. (G) The CCK-8 results on 1, 3, and 5 days (# and * indicate P < 0.05 in comparison with the control and blank groups, respectively).