Table 3. Activators of NF-κB identified by HTS assays examined by RT-qPCR.
There were 32 organic chemicals predicted to activate NF-κB selected for further study.
| Chemical | CASN | HTS Assay | AC50 (uM) | EFFICACY (fold increase) | Identified using the NF-κB Biomarker | Data Source | Validated as an NF-κB Activator in HeLa Cells by RT-qPCR1 |
|---|---|---|---|---|---|---|---|
| 1,10-Phenanthroline | 66-71-7 | ToxCast | 77.05706 | 3.163462 | NA | NA | Yes |
| 1,4-Dihydroxyanthracene-9,10-dione | 81-64-1 | ToxCast | 2.125469 | 1.86884 | NA | NA | Yes |
| 1-Amino-2-methylanthraquinone | 82-28-0 | ToxCast | 33.57287 | 2.952916 | NA | NA | Yes |
| Aloe-emodin | 481-72-1 | ToxCast | 9.564654 | 2.112953 | NA | NA | Yes |
| Apigenin | 520-36-5 | ToxCast | 24.31648 | 6.368289 | NA | NA | Yes |
| Biochanin A | 491-80-5 | ToxCast | 128.6124 | 3.190983 | NA | NA | Yes |
| C.I. Disperse Yellow 64 | 10319-14-9 | ToxCast | 26.37337 | 2.037707 | NA | NA | Yes |
| Chlorhexidine diacetate | 56-95-1 | ToxCast | 30.4311 | 4.095707 | NA | NA | Yes |
| Chlorophacinone | 3691-35-8 | ToxCast | 31.79655 | 4.897706 | NA | NA | Yes |
| Chlorpyrifos-methyl | 5598-13-0 | ToxCast | 9.022388 | 1.603474 | NA | NA | Yes |
| Chrysin | 480-40-0 | ToxCast | 11.41565 | 2.688455 | NA | NA | Yes |
| Flavone | 525-82-6 | ToxCast | 71.15899 | 5.433016 | NA | NA | Yes |
| Hexythiazox | 78587-05-0 | ToxCast | 26.59593 | 2.467522 | NA | NA | Yes |
| Phenyl 1-hydroxy-2-naphthoate | 132-54-7 | ToxCast | 81.67663 | 1.956113 | NA | NA | Yes |
| Prodiamine | 29091-21-2 | ToxCast | 34.63778 | 2.925536 | NA | NA | Yes |
| Quinalizarin | 81-61-8 | ToxCast | 27.32025 | 2.446469 | NA | NA | Yes |
| Tebufenpyrad | 119168-77-3 | ToxCast | 0.804216 | 2.914699 | NA | NA | Yes |
| 9-Ethyl-3-nitro-9H-carbazole | 86-20-4 | Tox21 | 61.64481 | 12.7423 | NA | NA | Yes |
| Aminoquinuride dihydrochloride | 5424-37-3 | Tox21 | 22.14763 | 15.76857 | NA | NA | No |
| Benzo(b)fluoranthene | 205-99-2 | Tox21 | 54.941 | 58.50674 | NA | NA | Yes |
| Carbocyanine | 605-91-4 | Tox21 | 19.73909 | 38.73972 | NA | NA | Yes |
| Cridanimod | 38609-97-1 | Tox21 | 20.51133 | 82.39178 | NA | NA | Yes |
| Dimethisoquin hydrochloride | 2773-92-4 | Tox21 | 11.53437 | 19.73928 | NA | NA | Yes |
| Falnidamol dihydrochloride | 1216920-18-1 | Tox21 | 13.44809 | 44.78139 | NA | NA | Yes |
| Lacidipine | 103890-78-4 | Tox21 | 23.01409 | 17.59346 | NA | NA | Yes |
| Lintitript | 136381-85-6 | Tox21 | 0.258222 | 17.89915 | NA | NA | Yes |
| Methyl 3-amino-5,6-dichloropyrazine-2-carboxylate | 1458-18-0 | Tox21 | 15.089 | 51.31506 | NA | NA | Yes |
| Mitoxantrone | 70476-82-3 | Tox21 | 0.425266 | 17.54468 | B-cell lymphoma cell line | GSE60408 | Yes |
| Thioridazine | 50-52-2 | Tox21 | 23.91446 | 14.72563 | PC-3 Cell Line, 9.8uM, 10uM | GSE5258 | Yes |
| Thioridazine hydrochloride | 130-61-0 | Tox21 | 25.39531 | 16.8285 | NA | NA | Yes |
| Triamterene | 396-01-0 | Tox21 | 23.91446 | 20.18841 | NA | NA | Yes |
| Vincamine | 1617-90-9 | Tox21 | 34.66543 | 13.88488 | PC-3 Cell Line, 11.2uM | GSE5258 | Yes |
All exposures were for 6 hrs at 50uM.
1Data from RT-qPCR studies in Fig 7.
Chemicals had to induce either CXCL1 or IL6 in wild-type cells but not NFKB1-null cells to be classified as confirmed.