Skip to main content
. 2022 Feb 2;8(5):eabl8920. doi: 10.1126/sciadv.abl8920

Fig. 1. Dexamethasone sensitizes to erastin-induced, but not to RSL3-induced, ferroptosis.

Fig. 1.

(A) HT1080 cells were treated for indicated times with 5 μM erastin and 1 μM Fer-1 with or without pretreatment of 1 μM dexamethasone for 12 hours. 7-AAD and annexin V were read out by fluorescence-activated cell sorting (FACS). (B) Quantification of data presented in (A) and repetitions of this experiment. (C) Still images of indicated times of a double-chamber time-lapse imaging and quantification of SYTOX-positive cells over time in HT1080 cells pretreated with dexamethasone as indicated. (D) HT1080 cells were induced to undergo ferroptosis by the GSH peroxidase 4 (GPX4) inhibitor RSL3 and assessed as in (A) after indicated times. Experiments were repeated at least three times, and representative images and FACS plots are shown. The graphs show means ± SD. Statistical analysis was performed using Student’s t test for each time point. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n.s., nonsignificant.