Fig. 4. Dexamethasone-mediated sensitization to erastin-induced ferroptosis is mediated by DPEP1.

(A) HT1080 cells were treated for 12 hours with dexamethasone before investigating protein expression levels of the GR and DPEP1. (B) Human kidney primary tubular epithelial cells (hTEC) were grown on Transwells and treated for 24 hours with dexamethasone before fluorescently labeling DPEP1. (C) HT1080 cells were treated 48 hours with siRNA against DPEP1, and knockdown efficacy was confirmed by Western blot. (D) HT1080 cells were treated with erastin and dexamethasone as indicated following the 48-hour pretreatment with an siRNA against DPEP1. Primary FACS plots and respective quantifications of indicated populations are demonstrated. The graphs show means ± SD. Statistical analysis was performed using Student’s t test for each time point. sc, scrambled; n.s., nonsignificant.