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. 2022 Feb 2;8(5):eabm0142. doi: 10.1126/sciadv.abm0142

Fig. 7. DPEP1-mediated leukocyte recruitment and tubular injury.

Fig. 7.

(A) Immunoblotting for LRP2, GPX4, ACSL4, and DPEP1 expression in whole-kidney tissue from Dpep1−/− and Dpep1+/+ mice 48 hours after renal IRI or sham operation. (B) Kidney IVM in LysMgfp/gfp mice at 90 min following LPS administration with or without cilastatin or LSALT peptide treatment. Scale bars, 100 μm. (C) Stationary GFP+ leukocytes/field in the kidney were quantified (versus LPS: cilastatin, *P = 0.01; LSALT, **P = 0.002; n = 4 to 5 per group, ANOVA with Dunnett’s post hoc test). (D) Kidney IVM in Dpep1+/+ and Dpep1−/− mice at 90 min following LPS administration. Labels: leukocytes (CD11b, red), capillaries (QTracker, blue), and tubules (autofluorescence, yellow-green). Scale bars, 100 μm. (E) Kidney IVM with SYTOX Red staining in LysMgfp/gfp mice 2 hours following LPS administration. Non-LPS (NT)–treated mice are shown as a control. Labels: leukocytes (LysM-GFP, bright green/yellow), tubules (autofluorescence, dark green), capillaries (QTracker, blue), and necrotic cells (SYTOX, red). Scale bars, 100 μm.