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. 2022 Jan 19;11:e75555. doi: 10.7554/eLife.75555

Figure 4. Optimizing and expanding the utility of split-ADAR2 deaminase domains (ADAR2-DD).

Figure 4.

(a) A split-ADAR2-DD(E488Q, N496F) was engineered and used to edit a GAC motif in the RAB7A transcript. Values represent mean ± SEM (n = 3). (b) 2D histograms comparing the transcriptome-wide A-to-G editing yields observed with full-length and split-ADAR2-DD(E488Q, N496F) constructs. Blue circles indicate the intended target UAG site within the RAB7A transcript. (c) A split-RESCUE was engineered and assayed for cytosine to uracil (C-to-U) editing of the RAB7A transcript. Values represent mean ± SEM (n = 3), quantified by NGS. (d) 2D histograms comparing the transcriptome-wide A-to-G and C-to-U editing yields observed with full-length and split RESCUE constructs. Blue circles indicate the intended target C site within the RAB7A transcript. All experiments were carried out in HEK293FT cells.

Figure 4—source data 1. Optimizing and expanding the utility of split-ADAR2 deaminase domains.