Figure 2.

CRTAC1 inhibits bladder cancer cell proliferation, migration, invasion and EMT process

(a-b) RT-qPCR was used to assess the overexpression or knockdown efficiency of CRTAC1 in T24 and 5637 cells respectively. (c) The viability of T24 and 5637 cells after CRTAC1 overexpression or knockdown was detected using CCK-8 assays. (d) The proliferative ability of T24 and 5637 cells after indicated transfection was detected using colony formation assays. (e) Wound healing assays were conducted to assess the migratory ability of T24 and 5637 cells after CRTAC1 overexpression or knockdown. (f) Transwell assays were performed to detect the invasion of T24 and 5637 cells overexpressing or silencing CRTAC1 expression. (g) Protein levels of EMT markers (E-cadherin, N-cadherin, Slug and Twist) were detected by western blotting in T24 and 5637 cells after the above transfection. *p < 0.05, **p < 0.01, ***p < 0.001.