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. 2021 Nov 24;12(2):9377–9389. doi: 10.1080/21655979.2021.1974645

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — CRTAC1 targets YY1 in bladder cancer cells

(a) Pearson correlation analysis was used to assess the expression correlation between CRTAC1 and YY1 in bladder cancer tissues. (b) The immunofluorescent staining was used to detect the expression and colocalization of CRTAC1 and YY1 in the nucleus of bladder cancer cells. (c) The interaction between CRTAC1 and YY1 was explored by ChIP and Co-IP assays. (d) Luciferase reporter assay was used to explore the binding between CRTAC1 and YY1 promoter. (e) YY1 expression in bladder cancer tissue samples was examined by RT-qPCR. (f) The mRNA and protein levels of YY1 in T24, 5637 and SVHUC-1 cells were examined by RT-qPCR and western blotting. (g) YY1 expression in bladder cancer cells after CRTAC1 overexpression or silencing. **p < 0.01, ***p < 0.001.