Skip to main content
. 2021 Nov 24;12(2):9377–9389. doi: 10.1080/21655979.2021.1974645

Figure 5.

Figure 5.

CRTAC1 inhibits malignant behaviors of bladder cancer cells by downregulating YY1

(a-b) The expression levels of CRTAC1 and YY1 in T24 cells after transfection of pcDNA3.1/CRTAC1, empty pcDNA3.1 vector or co-transfection of pcDNA3.1/CRTAC1 and pcDNA3.1/YY1 were examined by RT-qPCR. (c-d) CCK-8 and colony formation assays were performed to measure the viability and proliferation of T24 cells after the above transfection. (e-f) The migration and invasion of T24 cells after CRTAC1 and YY1 overexpression was assessed by wound healing and Transwell assays. (g) The protein levels EMT markers in T24 cells after the above transfection were detected by western blotting. *p < 0.05, **p < 0.01, ***p < 0.001.