Figure 3.

Downregulation of circ-PSEN1 alleviates ferroptosis of HG-induced ARPE19 cells. (a) The MTT assay was used to assess the cell survival rate of the cells treated with HG and si-circ-PSEN1 for 24 h. (b) PI positive cells were photographed and counted by fluorescence microscopy. DAPI was used for counter staining. The ARPE19 cells were treated with HG and si-circ-PSEN1 for 24 h. (c) TUNEL positive cells were captured and counted by fluorescence microscopy. The ARPE19 cells were treated with HG and si-circ-PSEN1 for 24 h. (d) The protein expressions of GPX4, SLC7A11, and TFR1 were detected by Western blotting 24 h after the indicated treatments. Data are representative of three experiments; **P < 0.01. HG, high glucose; ARPE19, adult retinal pigment epithelial cell line-19; PI, propidium iodide; DAPI, 4′,6-diamidino-2-phenylindole; TUNEL, TdT mediated dUTP Nick End Labeling