Figure 4.

miR-200b-3p is the target of circ-PSEN1. (a) The sequences of circ-PSEN1 WT, circ-PSEN1 MUT, and miR-200b-3p. (b) Luciferase activities of the ARPE19 cells were analyzed 24 h after the indicated treatments. (c) The levels of circ-PSEN1 enriched by negative control and biotin-labeled miR-200b-3p. (d) The expression levels of miR-200b-3p of the cells transfected with circ-PSEN1, si-circ-PSEN1, or their control. (e) The expression level of miR-200b-3p in the HG-treated ARPE19 cells. Data are representative of three experiments; **P < 0.01. HG, high glucose; ARPE19, adult retinal pigment epithelial cell line-19; WT, wild type; MUT, mutant type