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. 2021 Dec 6;12(2):10136–10149. doi: 10.1080/21655979.2021.2000745

Figure 1.

Figure 1.

Circ007293 was enriched in the exosomes from PTC patient serum and PTC cell lines. (a) Western blotting results of CD63 and CD81 expression in serum of PTC patients (T-serun; n = 40), serum of healthy controls (N-serum; n = 40), exosomes derived from the serum of PTC patients (T-exo; n = 40) or healthy controls (N-exo; n = 40). (b) The expression of circ007293 in T-exo or N-exo was analyzed using qRT-PCR. (c-e) TEM, NTA, and Western blotting were performed to confirm exosomes derived from Nthy-ori3-1 (Nthy-ori3-1-exo), TPC-1 (TPC-1-exo), or KTC-1 (KTC-1-exo) cells. (f) The expression of circ007293 in different PTC cell lines and exosomes derived from different cells was analyzed using qRT-PCR. (g) The expression of circ007293 in TPC-1 and KTC-1 cells transfected with oe-circ007293 or oe-NC was determined by qRT-PCR. (h) The expression of circ007293 in exosomes derived from TPC-1 and KTC-1 cells transfected with oe-circ007293 or oe-NC was analyzed using qRT-PCR. (i) The expression of circ007293 in TPC-1 and KTC-1 cells transfected with si-circ007293 or si-NC was analyzed using qRT-PCR. (j) The expression of circ007293 in exosomes derived from TPC-1 and KTC-1 cells transfected with si-circ007293 or si-NC was determined using qRT-PCR. **p < 0.01, and ***p < 0.001 compared to the control group