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. 2021 Dec 6;12(2):10136–10149. doi: 10.1080/21655979.2021.2000745

Figure 2.

Figure 2.

PTC cell-derived exosomal circ007293 promoted PTC cell proliferation, migration, invasion, and EMT. (a) Exosome uptake was assessed to confirm the uptake of PKH26-labeled oe-circ007293-exo (red) into recipient TPC-1 and KTC-1 cells. Magnification: ×200; scale bar: 50 μM. (b) Both oe-NC-exo and oe-circ007293-exo treatment increased the expression of circ007293 in TPC-1 and KTC-1 cells. (c) The viability of TPC-1 and KTC-1 cells co-cultured with oe-NC-exo or oe-circ007293-exo was evaluated using CCK-8 assay. (d) The migration of TPC-1 and KTC-1 cells co-cultured with oe-NC-exo or oe-circ007293-exo was analyzed using wound healing assay. Magnification: ×100; scale bar: 100 μM. (e) The invasion of TPC-1 and KTC-1 cells co-cultured with oe-NC-exo or oe-circ007293-exo was analyzed using transwell assay. Magnification: ×200; scale bar: 50 μM. (f) The levels of EMT protein markers (E-cadherin, N-cadherin, and vimentin) in TPC-1 and KTC-1 cells co-cultured with oe-NC-exo or oe-circ007293-exo were analyzed using Western blotting. *p < 0.05 and **p < 0.01 compared to the PBS group; ##p < 0.01 and ###p < 0.001 compared to the oe-NC-exo group