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. 2021 Dec 6;12(2):10136–10149. doi: 10.1080/21655979.2021.2000745

Figure 4.

Figure 4.

Circ007293 promoted PAX6 expression through acting as a ceRNA for miR-653-5p. (a) Subcellular fractionation for circ007293 in the nucleus and cytoplasm of TPC-1 and KTC-1 cells. (b) Left: Venn diagram showing miR-653-5p as the potential circ007293-targeting miRNA in the prediction results from Circinteractome and starBase. Right: Predicted binding sites of circ007293 and miR-653-5p, and miR-653-5p and PAX6 mRNA 3ʹUTR. (c) Evaluation of luciferase activity in TPC-1 and KTC-1 cells co-transfected with circ007293-WT or MUT reporter vectors and miR-653-5p or miR-NC. (d) Evaluation of luciferase activity in TPC-1 and KTC-1 cells co-transfected with PAX6-WT or MUT reporter vectors and miR-653-5p or miR-NC. (e) The expression of miR-653-5p in TPC-1 and KTC-1 cells transfected with oe-circ007293 or oe-NC was analyzed using qRT-PCR. (f) The mRNA and protein expression levels of PAX6 in TPC-1 and KTC-1 cells transfected with oe-circ007293 or oe-NC were determined by qRT-PCR and Western blotting, respectively. (g) The mRNA and protein expression levles of PAX6 in TPC-1 and KTC-1 cells co-transfected with miR-653-5p mimic and oe-circ007293 were analyzed using qRT-PCR and Western blotting, respectively. **p < 0.01 and ***p < 0.001