Figure 1.

Overexpression of circATRNL1 indicated the unfavorable prognosis of osteosarcoma patients. (a) schematic diagram illustrated the genesis of circATRNL1 from the ATRNL1 gene loci through backsplicing. (b) screening analysis using the RT-PCR discovered the potential up/down-expressed circRNA in the osteosarcoma tissue and normal tissue. (c) sanger sequencing revealed the junction sites of circATRNL1 using the cDNA in the cells. (d) act D administration and (e) RNase R administration were performed to detect the stability of circATRNL1 and ATRNL1 mRNA. the levels of RNA were measured by RT-PCR. (f) in the enrolled osteosarcoma patients’ specimens, circATRNL1 expression was detected using RT-PCR. (g) clinically, the patients with high-expression of circATRNL1 demonstrated a lower prognosis than who with low-expression of circATRNL1. two-group comparison was calculated by student’s t-test. experiments were performed in triplicate. multiple group comparison was calculated by one-way analysis of variance (ANOVA). data are presented as mean ± SD. *p < 0.05, **p < 0.01