Figure 4.

MiR-409-3p targeted LDHA axis in the osteosarcoma cells. (a) screening found that LDHA acted as the most promising target of miR-409-3p using the bioinformatics predictive manners (TARGETSCAN, miRanda, PITA). (b) mutation and wild type of LDHA were established for the luciferase assay. (c) luciferase gene reporter assay indicated the activity in the co-transfection of miR-409-3p and LDHA wild type. (d) RT-PCR illustrated the LDHA mRNA in Saos-2 cells transfected with circATRNL1 or/and miR-409-3p. (e) western blot analysis detected the LDHA protein levels in the saos-2 cells transfected with circATRNL1 or/and miR-409-3p. data are presented as mean ± SD. two-group comparison was calculated by student’s t-test. experiments were performed in triplicate. *p < 0.05, **p < 0.01