Figure 2.

The deficiency of miR-934 inhibited cell proliferation and induced cell cycle arrest in colorectal cancer cell lines. MiR-934 inhibitor (100 pmol) or NC inhibitor (100 pmol) was transfected into HCT116 and SW480 cells. (a) The transfection efficiency was subsequently detected by qRT-PCR. (b) Cell viability was determined by MTT assay. (c) Flow cytometry was performed to determine cell cycle in CRC cell lines. (d&e) The protein levels of Ki-67 and cyclin D1 in CRC cell lines was analyzed by western-blot. Bars and error bars represent mean values and the corresponding SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001