Figure 4.

miR934 knockdown depressed angiogenesis in colorectal cancer cell lines. HCT116 and SW480 cells were transfected with miR-934 inhibitor (100 pmol) and NC inhibitor (100 pmol). After transfection, protein levels (a) and supernatant content (b) of VEGF were subsequently measured by western-blot and ELISA. (c) The mRNA levels of miR-934 in cell supernatants were determined by real-time PCR. (d) The representative images of tube formation in HUVECs were captured after the co-culture in TCM of SW480 and HCT116 cells, which was followed with calculation of tube formation ratio. Scale bar: 200 μm. Bars and error bars re present mean values and the corresponding SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001