Figure 5.

BTG2 was a target of miR-934. (a) The mutant and original site in 3ʹUTR of BTG2. (b) The dual luciferase reporter assay was performed to validate that BTG2 was the target gene of miR-934. (c) western-blot analysis was conducted to measure the protein level of BTG2 in HCT116 and SW480 cell line after transfection of miR-934 inhibitor (100 pmol), miR-934 mimics (100 pmol) and their negative control. Bars and error bars represent mean values and the corresponding SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001