Figure 3.

OIP5-AS1 overexpression weakened lung injury in vivo. Ad-GFP or Ad-OIP5-AS1 was injected into the SD rats via tail vein. CLP surgery was used to induce septic rat model. 48 hours after the construction of the mode, the lung tissues were isolated and subjected to further experiments. (a) The examination of the pathological changes in rat lung tissue of each group was conducted by HE staining method. (b) The dry/wet method was adopted for testing pulmonary edema. (c) ICH was used to evaluate MPO-labeled polymorphonuclear neutrophils (PMN). (d) The relative expression of apoptosis-related proteins Caspase3, Bax and Bcl2 related by apoptosis in each group was examined applying the Western blot method. (e) Western blot method was performed for the examination of the relative expression of SIRT1, p-NF-κB and iNOS. (f) SIRT1 expression in lung tissue was measured by immunohistochemical staining. (g) ELISA was applied for measuring the levels of related inflammatory factors IL-1β, TNFα and IL-6 in the lung homogenate supernatant. ** P < 0.01, *** P < 0.001. N = 5