Figure 1.

RBM38 is downregulated in in-vitro models of myocardial hypertrophy

a-b: Assessment of Myocardial hypertrophy markers ANF and Myh7 expressions by western blot after stimulation of H9C2 cells with PE, TNF-α, and AngII. (a). The Myh7 and ANF expressions were increased following PE, TNF-α, and AngII stimulation compared to the controls.b: Increased relative Myh7 mRNA expression following H9C2 cells stimulation with PE, TNF-α, and AngII as confirmed by RT-qPCR.c: Increased relative ANF mRNA expression following H9C2 cells stimulation with PE, TNF-α, and AngII as confirmed by RT-qPCR.d: H&E staining and E: Rhodamine-labeled phalloidin staining assays showing increased relative cell surface area in PE, TNF-α, and Ang II-treated cells.f: Western blot representation of reduced RBM38 protein expression in control, PE-, TNF-α, and the Ang II-treated cells compared to the control group.g: Rbm38 mRNA expression assessment showed reduced RBM38 mRNA levels in the PE, TNF-α, and Ang II treatment. (*p < 0.01)