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DHA alleviated morphine-induced inflammatory response in BV-2 cells
BV-2 cells were treated with DHA (1, 5, 10, and 20 μM) or minocycline (10 μM) 30 min prior to morphine induction. (a-c) The production of inflammatory cytokines (IL-1β, IL-6, and TNF-α) were detected by ELISA assays. (d-f) The expression of inflammatory cytokines (IL-1β, IL-6, and TNF-α) were detected by quantitative real-time PCR. *** p < 0.001 vs control group; #, ##, ### p < 0.05, 0.01, 0.001 vs morphine group.
