Figure 2.
M2-EVs reduced the inflammatory response in UC mice. The murine model of UC was established by DSS induction. M2-EV or GW4869-treated conditioned medium was injected into UC mice, and the colon tissues were collected on the 11th day. a: HE staining and UC score. b: Colon length and weight of mice in each group. c: DAI score of mice in each group. d: Cell apoptosis in colon tissues was measured using TUNEL staining. e: The levels of apoptosis-related factors (Bax and Bcl-2) were determined using RT-qPCR. f: The contents of inflammatory cytokines (TNF-α, IL-1β, MCP-1, and IL-10) were determined using ELISA. N = 6. Data in panels B/C/D/F are presented as mean ± standard deviation. Data in panels A-E were analyzed using one-way ANOVA, and data in panel F were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, **p < 0.01