Figure 5.

M2-EVs protected UC cells by carrying MEG3. M2 macrophages were transfected with LV-MEG3 or LV-NC, and then M2-EVs were isolated. a: MEG3 expression in M2 macrophages was detected using RT-qPCR. b: MEG3 expression in M2-EVs was detected using RT-qPCR. LPS-induced YAMC cells were treated with the above M2-EVs. c: MEG3 expression in YAMC cells was detected using RT-qPCR. d-e: Cell viability and apoptosis were measured using CCK-8 assay (d) and flow cytometry (e). f: Bax and Bcl-2 mRNA levels were determined using RT-qPCR. G: The contents of inflammatory cytokines (TNF-α, IL-1β, MCP-1, and IL-10) were determined using ELISA. The experiment was repeated 3 times independently. Data are presented as mean ± standard deviation. Data in panels A-E were analyzed using one-way ANOVA, and data in panels F/G were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, **p < 0.01