Figure 7.

miR-20b-5p overexpression attenuated the protective effect of M2-EVs on UC cells by reducing CREB1 transcription. miR-20b-5p mimic was transfected into M2-EVs-treated YAMC cells, with mimic NC as control. a-b: miR-20b-5p and CREB1 expression in cells were detected using RT-qPCR. c-d: Cell viability and apoptosis were measured using CCK-8 assay (c) and flow cytometry (d). e: Bax and Bcl-2 mRNA levels were determined using RT-qPCR. f: The contents of inflammatory cytokines (TNF-α, IL-1β, MCP-1, and IL-10) were determined using ELISA. The experiment was repeated 3 times independently. Data are presented as mean ± standard deviation. Data in panels A-D were analyzed using one-way ANOVA, and data in panels E/F were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, **p < 0.01