Figure 8.

miR-20b-5p overexpression attenuated the protective effect of M2-EVs on UC mice by reducing CREB1 transcription. miR-20b-5p agomir was injected into mice in the M2-EVs group via tail vein, with control agomir as negative control. The colon tissues were collected on the 11th day. a-b: miR-20b-5p and CREB1 expressions were determined using RT-qPCR. c: HE staining and colitis score. d: Colon length and weight of mice in each group. e: DAI score of mice in each group. f: Apoptosis rate detected using TUNEL staining. g: Apoptosis-related factors (Bax and Bcl-2) detected using RT-qPCR. h: Inflammatory cytokines (TNF-α, IL-1β, MCP-1, and IL-10) determined using ELISA. N = 6. The experiment was repeated 3 times independently. Data in panels D/E/F/H are presented as mean ± standard deviation. Data in panels A-G were analyzed using one-way ANOVA, and data in panel H were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, **p < 0.01