Figure 2.

TOP2A enhances HCC cell viability, invasion, and migration. (a, b) Knockdown of TOP2A in MHCC97H cells and upregulation of TOP2Ain Hep3B cells were analyzed through Western blot (a) and RT-qPCR (b) (c) CCK-8 assay results showed that TOP2A enhanced the viability of MHCC97H and Hep3B cells. D. Apoptotic proteins were detected after changing TOP2A expression. E. Proliferation of MHCC97H cells and Hep3B cells after changing TOP2A expression was detected by subcutaneous tumor formation in nude mice; Ki-67 expression was also detected, Magnification, 400 × (f) The migration capacity was determined by scratch assays and by quantifying open area percentage. Magnification, 100 × . (g) Results of transwell Matrigel invasion assays following knockdown or overexpression of TOP2A in HCC cells are shown. Magnification, 200 × . (f) Lung tissues showing metastatic foci of MHCC97H-GFP and Hep3B -GFP. Typical images for lung tissue sections of every group and pulmonary metastasis rates are presented. Error bars indicate SD. *P < 0.05, **P < 0.01, ***P < 0.001