Figure 3.

TOP2A promotes EMT by upregulating Snail expression. (a, b) Western blot (a) and RT-qPCR (b) results show the alterations of EMT marker levels after stable TOP2A downregulation and upregulation in HCC cells. (c,d) Role of TOP2A in Snail, Slug, Zeb1/2, and Twist levels was analyzed through Western blot (c) and RT-qPCR (d). (e) Western blot results showed upregulation of Snail in MHCC97H cells and downregulation of Snail in Hep3B cells. (f) N-cadherin and E-cadherin expressions in HCC cells after transfection with indicated vectors were assessed. Error bars indicate SD. *P < 0.05, **P < 0.01, ***P < 0.001