Figure 4.

TOP2A enhances Snail expression by regulating the p-ERK1/2/p-SMAD2(S425/250/255) signaling pathway. (a) The activation of several signaling molecules was detected after knockdown or overexpression of TOP2A in HCC cells. (b) Hep3B cells after exposure to chemical inhibitors, including LY3214996 (ERK1/2), LY294002 (PI3K/AKT), SP600125 (SAPK/JNK), and SB203580 (P38) for a 60-min period. Snail expression in HCC cells was analyzed. (c,d) Migration and invasion capability were determined after exposure of Hep3B-TOP2A cells to chemical inhibitors. Error bars indicate SD. *P < 0.05, **P < 0.01, ***P < 0.001