Skip to main content
. 2021 Dec 7;12(2):12107–12122. doi: 10.1080/21655979.2021.2003941

Figure 5.

Figure 5.

LINC00857 modulates CRC progression through sponing miR-150-5p. (a) miR-150-5p inhibitor suppressed miR-150-5p level as detected by qRT-PCR. (b) & (c) miR-150-5p inhibitor rescued the inhibition of LINC00857 silencing on proliferation of SW480 and HCT116 cells, as examined by CCK-8 assay. (d) miR-150-5p inhibitor rescued the inhibitory effect of LINC00857 silencing on colony formation of SW480 and HCT116 cells. (e) Annexin V/PI staining for apoptosis analysis. Cells in quadrat 2 and 3 were considered as apoptotic cells. (f & g) miR-150-5p inhibitor rescued the inhibitory effect of LINC00857 silencing on the migration (f) and invasion (g) of SW480 and HCT116 cells. Scale Bar: 50 µM. For all the above experiments: sh-NC: cell stabling expressing control shRNA; sh-LINC00857: cells stably expressing sh-LINC00857; sh-LINC00857+ miR-150-5p inhibitor: cells stably expressing sh-LINC00857 were transfected with miR-150-5p inhibitor. *P < 0.05, **P < 0.01, ***P < 0.001