Figure 2.

MiR-889-3p represses the proliferation and EMT of LC cells

A. After transfection of miR-889-3p or in-miR-889-3p, miR-889-3p in A549 cells detected by qPCR; B. The growth rate of cells after transfection of miR-889-3p or in-miR-889-3p detected by CCK-8 method; C. Cell proliferation after transfection with miR-889-3p or in-miR-889-3p examined by plate cloning; D. Migration and invasion of cells after transfection with miR-889-3p or in-miR-889-3p detected by Transwell; E. Flow cytometry employed to detect apoptosis after transfection with miR-889-3p or in-miR-889-3p; F. Detection via qPCR for EMT-related molecules E-cadherin and N-cadherin. G. Western Blot analysis of pAkt, Perk, N-cadherin and Vimentin protein levels. The data in the figures were all measurement data in the form of mean ± SD. * vs the miR-NC, P < 0.05; + vs the in-miR-NC, P < 0.05.