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. 2021 Nov 27;12(2):10945–10958. doi: 10.1080/21655979.2021.2000283

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — HIPK1 overexpression motivates the growth and metastasis of LC cells

A. qPCR to detect the expression of HIPK1 after elevation of HIPK1; B/C. CCK-8 and plate cloning to detect the proliferation of cells after elevated HIPK1; D. Transwell to detect the invasion and migration abilities of cells after elevated HIPK1; E. Cell apoptosis after elevated HIPK1 detected by flow cytometry; F. EMT-related molecules E-cadherin and N-cadherin detected by PCR; G. Western Blot analysis of pAkt, Perk, N-cadherin and Vimentin protein levels. The data in the figures were all measurement data in the form of mean ± SD. & vs the oe-NC, P < 0.05.