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The mRNA expression of B7-H3 isoforms in de novo AML patients and controls. RT-PCR with B7-H3-specific primer3 was used to detect mRNA expression of B7-H3 isoforms in AML patients and controls followed by analysis by ROC curve. (a-b) The ordinate represents gray values of 4Ig B7-H3 and 2Ig B7-H3/gray value of β-actin, respectively. (c-d) The ROC curve analysis of 4Ig B7-H3 and 2Ig B7-H3 mRNA expression. AUC, area under the ROC curve
