Table 1.
Primer sequences for B7-H3 isoforms
| Primers | Products | Sequences (5ʹ-3ʹ) |
|---|---|---|
| Primer 1 | B7-H3 (134bp) [32] | F:5ʹ-CTCTGCCTTCTCACCTCTTTG-3ʹ R:5ʹ-CCTTGAGGGAGGAACTTTATC-3’ |
| Primer 2 | 4Ig B7-H3 (380bp) [25] | F:5ʹ-CTCACGAAGCAGGTGAAGCTGCC-3ʹ R:5ʹ-ACCTACAGCTGCTGGTGCGCAA-3’ |
| Primer 3 |
B7-H3 isoforms (1444bp and 790bp) [5] |
F:5ʹ-CACAGGAAGATGCTGCGTCG-3ʹ R:5ʹ-CAATGAGACAGACAGACAGC-3’ |
| Primer 4 |
B7-H3 isoforms (1572bp and 918bp) [6] |
F:5ʹ-CAGCCCTGGCATGGGTGTGCAT-3ʹ R:5ʹ-CCATCATCTTCTTTGCTGTCAGAG-3’ |
| Primer 5 | β-actin (205bp) [33] | F:5ʹ-TGACGTGGACATCCGCAAAG-3ʹ R:5ʹ-CTGGAAGGTGGACAGCGAGG-3’ |