Figure 3.

Evaluation the binding site between LINC00240 and miR-338-5p. (a) The binding site between LINC00240 and miR-338-5p based on bioinformatics analysis. (b) Increased expression level of miR-338-5p after LINC00240 knockdown detected by qRT-PCR. (c) Dual luciferase reporter assay using empty luciferase vector (luci-LINC00240-NC), wild type reporter with binding site (luci-LINC00240-WT), and the vector containing mutated binding sequence (luci-LINC00240-MUT) in the presence of miR-338-5p mimic. (n = 3, **P < 0.01). (d and e) Downregulation of miR-338-5p expression in GC tissue from TCGA datasets (d) and the GC tissues collected in this study (e). (f) Negative correlation between LINC00240 and miR-338-5p expression in GC tissues collected in this study (R² = 0.5086, p < 0.001)