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. 2021 Nov 27;12(2):10023–10036. doi: 10.1080/21655979.2021.1994721

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — METTL3 promoted m6A methylation in pri-miR-20a-5p to upregulate miR-20a-5p expression pattern. ECA109 and TE-1 cells were transfected with sh-METTL3 with sh-NC as the control. The role of METTL3 in the epigenetic regulation of miR-20a-5p was observed. A: m6A modification level in total RNA of ECA109 and TE-1 cells was detected via m6A assay kits; B: Enrichment of DGCR8 and m6A in pri-miR-20a-5p in ECA109 and TE-1 cells was detected via RNA co-immunoprecipitation assay; C: the miR-20a-5p expression pattern in ECA109 and TE-1 cells was detected via qRT-PCR. Cell experiment was conducted 3 times independently. All data were measurement data and represented as mean ± SD. Data in figures A and C were analyzed using one-way ANOVA and data in figure B were analyzed using two-way ANOVA. After analysis, data were verified by Tukey’s multiple comparison test. *** P < 0.001