Figure 4.

miR-20a-5p promoted EMT, invasion, and migration of ESCA cells. ESCA cells with a poor expression pattern of METTL3 were transfected with miR-20a-5p mimic, with mimic NC as the control. EMT process, cell proliferation, migration, and invasion were observed. A: miR-20a-5p expression pattern was detected via qRT-PCR; B: Positive expression patterns of N-cadherin and E-cadherin were detected via immunofluorescence; D: Cell invasion and migration potentials were detected via Transwell assays; Cell experiment was conducted 3 times independently. All data were measurement data and represented as mean ± SD. Data in figures A and C were analyzed using one-way ANOVA and data in figure B were analyzed using two-way ANOVA. After analysis, data were verified by Tukey’s multiple comparison test. *** P < 0.001