Figure 6.

METTL3 downregulation inhibited tumor growth and metastasis in vivo. Nude mice were subcutaneously or caudal-vein injected with sh-METTL3-transfected ECA109 cells with sh-NC as the control. A: Expression patterns of METTL3, miR-20a-5p, and NFIC were detected via qRT-PCR; B: Volumes and weights of representative tumors were measured; C: Expression patterns of N-cadherin and E-cadherin were detected via western blot; D: Lung metastases were observed via HE staining. N = 6, all data were measurement data and represented as mean ± SD. Data in figures A, B (tumor weight), D were analyzed using one-way ANOVA, and data in figures B (tumor volume) and C were analyzed using two-way ANOVA. After analysis, data were checked by Tukey’s multiple comparison test. *** P < 0.001