Figure 5.

Overexpression of miR-340-3p reverses the effects of PSP on survival and glucose and TG metabolism in the insulin-resistant L6 myotubes. (a) The survival of PA-treated L6 myotubes as assessed by CCK8 assay after transfection with miR-340-3p mimic and inhibitor for 48 h. (b) The glucose concentration in the L6 myotubes supernatant as measured by glucose detect kit after stimulation with 100 nM insulin and after transfection with miR-340-3p mimic and inhibitor at 48 h. Glucose uptake efficiency was negatively correlated with glucose concentration in the supernatant. (c) The GLUT-4 expression in L6 myotubes as measured by qRT-PCR. (d) The effects of PSP on lipid accumulation as assessed by Oil Red O staining (left, ×400). The TG concentration in the L6 myotubes were measured by TG detect kit after treatment for 24 h (right). (e) The levels of IL-1β, IL-6, and TNF-α in the cell supernatant as measured by ELISA. ***P < 0.001. TG: triglyceride; PSP: Polygonatum sibiricum polysaccharide; PA: palmitic acid; NC: negative control; CCK8: Cell Counting Kit-8; NC: negative control