Figure 3.

Upregulation of miR-140-5p alleviated LPS-induced chondrocyte pyroptosis in vitro. mouse chondrocytes were isolated and cultured in vitro. the cell model of OA was established by LPS induction and the model cells were transfected with miR-140-5p-mimic, with miR-NC as the control. A: miR-140-5p expression in cells was detected using qRT-PCR. B: cell viability was detected using CCK-8 assay. C: Pyroptosis was measured using calcein-AM/PI staining. D: GSDMD-N protein level was detected using western blot. E: cleaved caspase-1, IL-1β, IL-18, and ROS levels were detected using the kits. the cell experiment was repeated 3 times independently. the measurement data are expressed as mean ± standard deviation. data in panels A/C/D/E were analyzed using one-way ANOVA, and data in panel B were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, ***p < 0. 001